• UGI USER
  • Jul 30, 2024
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BREAKTHROUGH GENE-EDITOR BY INDIAN SCIENTISTS

Why in the news?

Indian scientists develop a precise CRISPR system using FnCas9, showing improved genome editing and potential for affordable therapeutics.

source:medium

About Enhanced CRISPR System with FnCas9:

  • CSIR-IGIB and L.V. Prasad Eye Institute developed a precise genome-editing system using FnCas9 enzyme.
  • FnCas9 edits genomes more efficiently with fewer off-target effects compared to SpCas9.
  • Modified FnCas9 shows higher binding affinity to PAM sequence and greater flexibility for hard-to-access genome regions.
What is Genome and Genome Sequencing?

  • Genome: Complete set of an organism’s DNA, including all genes; human genome has over 3 billion base pairs.
  • Genome Sequencing: Determines DNA nucleotide order (Adenine, Cytosine, Guanine, Thymine).
  • Applications: Advances in medical science, disease management, precision medicine.
  • Importance: Understanding genome function and non-gene regions.

What is Genome Editing?

  • Definition: Genome editing alters an organism’s DNA, adding, removing, or altering genetic material at specific genome locations.
  • CRISPR System: Advanced, precise tool for targeted genome interventions.

Categories:

  • SDN 1: Small insertions/deletions, no foreign DNA.
  • SDN 2: Specific changes using DNA template, no foreign DNA.
  • SDN 3: Involves foreign DNA, similar to GMO development.

About Clustered Regularly Interspaced Short Palindromic Repeats:

  • CRISPR : Gene-editing technology that alters DNA sequences by cutting, adding, or removing genetic material.
  • Development: Adapted from bacterial immune systems that cut viral DNA.
  • Components: Cas9 enzyme (molecular scissors) and guide RNA (directs Cas9 to DNA).
  • Applications: Potential to treat genetic conditions like cancer, hepatitis B, and high cholesterol.